RNA Viruses: A Practical Approach

RNA Viruses is wide ranging in scope, from core methods to emerging technology such as reverse genetics and retrovirus vectors. Chapter one covers the fundamentals of investigating RNA virus genome structure at a molecular level. Chapters two and three describe techniques for mutagenesis of RNA genomes and analysis of transcription. Chapter four deals with RNA virus-encoded proteinases, and important aspect of the control of RNA virus gene expression. Chapter five considers retrovirus oncogenesis and chapter six analysis of RNA virus quasispecies. Chapter seven describes systems for investigation of in vitro replication of positive-stranded viruses and chapter eight the packaging of RNA virus genomes. In addition to the technical aspects of reverse genetics and retrovirus vectors, both of the final two chapters also consider ethical aspects of these new technologies.

All protocols are provided in easy to follow step format with full listing of equipment and reagents required. These methods really work. To encourage success, the authors also list useful hints and tips, discuss potential problems and their solution and present typical data. No other methods manual in this field is as comprehensive in its support. The book is therefore highly recommended for all researchers who work on RNA viruses.

The book contains black-and-white illustrations.