Protein Structure: A Practical Approach

Protein Structure: A Practical Approach gives general methods for determining protein structure that will be very widely applicable, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry and ultracentrifugation. This second edition has been fully updated to embrace the most recent developments in the field, and will be invaluable to all involved in protein research.

Gene A. Homandberg

This second edition manual of methods in protein structure studies includes, but is not limited to, chapters on mass determination by gel analysis and mass spectrometry, immunologic detection of proteins of known sequence, identification of post-translational modifications, peptide mapping, determination of disulfide bonding patterns, analysis of protein conformation by gel electrophoresis, analysis of hydrodynamic properties, use of spectroscopy to characterize protein conformation and conformational changes, immunologic analysis of protein conformation, and stabilization of protein by solvents. This manual is meant to update common methods given in the first edition as well as introduce newer chapters on mass spectrometry and ultracentrifugation, while still retaining the original emphasis on simple and common methods. The audience should include not only student researchers, since it will be of great value in experimental design, but also experienced protein chemists who need an update on methods already in use. Also, molecular biologists with less experience in these methods should find these protocols easy to use and very practical. The features of the manual include ample and relevant diagrams, protocol boxes with easy-to-read detailed instructions, and concisely written general descriptions. A list of suppliers is also included. This manual is highly recommended for anyone using protein structure targeted methods. It will be highly useful to anyone, regardless of their experience, because the methods are updated and are presented very clearly. The diverse, relevant, and important topics covered also add to the value of this book.