PCR Protocols: A Guide to Methods and Applications

Optimization Of Pcrs / Michael A. Innis And David H. Gelfand -- Amplification Of Genomic Dna / Randall K. Saiki -- Amplification Of Rna / Ernest S. Kawasaki -- Race : Rapid Amplification Of Cdna Ends / Michael A. Frohman -- Degenerate Primers For Dna Amplification / Teresa Compton -- Cdna Cloning Using Degenerate Primers / Cheng Chi Lee And C. Thomas Caskey -- Pcr With 7-deaza-2'-deoxyguanosine Triphosphate / Michael A. Innis -- Competitive Pcr For Quantitation Of Mrna / Gary Gilliland, Steven Perrin, And H. Franklin Bunn -- Quantitative Pcr / Alice M. Wang And David F. Mark -- Production Of Single-stranded Dna By Asymmetric Pcr / Peter C. Mccabe -- Cloning With Pcr / Stephen J. Scharf -- Oligonucleotide Ligation Assay / Ulf Landegren, Robert Kaiser, And Leroy Hood. Nonisotopically Labeled Probes And Primers / Corey Levenson And Chu-an Chang -- Incorporation Of Biotinylated Dutp / Y.-m. Dennis Lo, Wajahat Z. Mehal, And Kenneth A. Fleming -- Nonisotopic Detection Of Pcr Products / Rhea Helmuth -- Thermostable Dna Polymerases / David H. Gelfand And Thomas J. White -- Procedures To Minimize Pcr-product Carry-over / Shirley Kwok -- Sample Preparation From Blood, Cells, And Other Fluids / Ernest S. Kawasaki -- Sample Preparation From Paraffin-embedded Tissues / Deann K. Wright And M. Michele Manos -- Amplifying Ancient Dna / Svante Pääbo -- In Vitro Transcription Of Pcr Templates / Michael J. Holland And Michael A. Innis -- Recombinant Pcr / Russell Higuchi -- Dnase I Footprinting / Barbara Krummel -- Sequencing With Taq Dna Polymerase / Mary Ann D. Brow. Direct Sequencing With The Aid Of Phage Promoters / Steve S. Sommer [and Others] -- Identifying Dna Polymorphisms By Denaturing Gradient Gel Electrophoresis / Val C. Sheffield, David R. Cox, And Richard M. Meyers -- Amplification Of Flanking Sequences By Inverse Pcr / Howard Ochman [and Others] -- Detection Of Homologous Recombinants / Michael A. Frohman And Gail R. Martin -- Rna Processing : Apo-b / Lyn M. Powell -- A Transcription-based Amplification System / T.r. Gingeras [and Others] -- Screening Of [lambda] Gt11 Libraries / Kenneth D. Friedman [and Others] -- Hla Dna Typing / Henry A. Erlich And Teodorica L. Bugawan -- Multiplex Pcr For The Diagnosis Of Duchenne Muscular Dystrophy / Jeffrey S. Chamberlain [and Others] -- Isolation Of Dna From Fungal Mycelia And Single Spores / Steven B. Lee And John W. Taylor -- Genetic Prediction Of Hemophilia A / Scott C. Kogan And Jane Gitschier. Edited By Michael A. Innis ... [et Al.]. Includes Bibliographical References And Index.