PCR Applications: Protocols for Functional Genomics

Pt. 1. Key Concepts For Pcr-- Ch. 1. Optimization Of Pcr: Conversations Between Michael And David-- Ch. 2. The Convergence Of Pcr, Computers, And The Human Genome Project: Past, Present, And Future-- Ch. 3. Thermostable Dna Polymerases: An Update-- Ch. 4. Musings On Microbial Genomes-- Ch. 5. Statistical Refinement Of Primer Design Parameters-- Ch. 6. Multiplex Pcr: Optimization Guidelines-- Ch. 7. The Use Of Immobilized Mismatch Binding Protein For The Optimization Of Pcr Fidelity. Ch. 8. A New Generation Of Pcr Instruments And Nucleic Acid Concentration Systems-- Ch. 9. Sequencing Pcr Products-- Ch. 10. Recent Advances In High-temperature Reverse Transcription And Pcr-- Ch. 11. Viral Genotyping By A Quantitative Point Mutation Assay: Application To Hiv-1 Drug Resistance-- Ch. 12. In Situ Pcr-- Pt. 2. Quantitative Pcr-- Ch. 13. Standards For Pcr Assays-- Ch. 14. Rapid Thermal Cycling And Pcr Kinetics-- Ch. 15. Kinetics Of Competitive Reverse Transcriptase-pcr. Ch. 16. Kinetic Pcr Analysis Using A Ccd Camera And Without Using Oligonucleotide Probes-- Ch. 17. Quantification Of Telomerase Activity Using Telomeric Repeat Amplification Protocol-- Pt. 3. Gene Discovery-- Ch. 18. Differential Display-- Ch. 19. Single-cell Cdna Libraries-- Ch. 20. Whole Cell Assays-- Ch. 21. Screening Differentially Displayed Pcr Products By Single-strand Conformation Polymorphism Gels-- Ch. 22. Microsatellite Protocols-- Ch. 23. Real-time Quantitative Pcr: Uses In Discovery Research. Ch. 24. Homology Cloning: A Molecular Taxonomy Of The Archaea-- Ch. 25. Cloning Mammalian Homologs Of Drosophila Genes-- Ch. 26. Cloning Human Homologs Of Yeast Genes-- Pt. 4. Genomics And Expression Profiling-- Ch. 27. Cellular Transcriptome Analysis Using A Kinetic Pcr Assay-- Ch. 28. Parallel Analysis With Biological Chips-- Ch. 29. High-density Cdna Grids For Hybridization Fingerprinting Experiments-- Ch. 30. Comparative Genomics Hybridization-- Ch. 31. Genetic Footprinting And Functional Maps Of The Yeast Genome. Ch. 32. Molecular Analysis Of Microdissected Tissue: Laser Capture Microdissection-- Ch. 33. Amplified Fragmant Length Polymorphism: Studies On Plant Development-- Ch. 34. A Florescent, Multiplex Solid-phase Minisequencing Method For Genotyping Cytochrome P450 Genes-- Ch. 35. The Cleavase I Enzyme For Mutation And Polymorphism Scanning. Edited By Michael A. Innis, David H. Gelfand, John J. Sninsky. Includes Bibliographical References And Index.