Nuclear Structure And Function

Part I. Cell Fractionation Preparation And Characterization Of Probes. 1. Isolation And Characterization Of Karyoskeletal Protein-enriched Fractions From Vertebrate Livers -- 2. Preparation Of Karyoskeletal Protein-enriched Fractions From Drosophila Melanogaster Cells And Tissues -- 3. Isolation Of Nuclei And Nucleoli From The Yeast Saccharomyces Cerevisiae -- Part Ii. Structural Analysis Of The Interphase Nucleus. 4. Determining Nuclear Structure Using The Fluorescence Light Microscope -- 5. Localization Of Single Nuclear Pore Complexes By Confocal Laser Scanning Microscopy And Analysis Of Their Distribution -- 6. Nuclear Ultrastructure: Transmission Electron Microscopy And Image Analysis -- 7. Three-dimensional Surface Structure Analysis Of The Nucleus -- 8. Scanning Transmission Electron Microscopy Of Nuclear Structures -- Part Iii. Chromatin And Subnuclear Structures. 9. Electron Microscopic Imaging Of Chromatin With Nucleosome Resolution -- 10. Mapping Three-dimensional Chromosome Architecture In Situ -- 11. Structural Analysis Of Meiotic Chromosomes And Synaptonemal Complexes In Higher Vertebrates -- 12. Genetic And Morphological Approaches For The Analysis Of Meiotic Chromosomes In Yeast -- 13. Mapping Proteins To Nuclear Pore Complexes By Immmunogold Electron Microscopy -- 14. Methods Used To Study Structure And Function Of The Nucleolus -- 15. Identification Of Base-unpairing Region-binding Proteins And Characterization Of Their In Vivo Binding Sequences -- Part Iv. Nuclear Assembly/disassembly In Cell-free Systems. 16. Cell-free Nuclear Reassembly In Mammalian Mitotic Homogenates -- 17. Analysis Of Nuclear Envelope Assembly Using Extracts Of Xenopus Eggs -- 18. Cell-free Nuclear Assembly And Disassembly In Drosophila -- 19. Methods For Studying In Vitro Assembly Of Male Pronuclei Using Oocyte Extracts From Marine Invertebrates: Sea Urchins And Surf Clams -- Part V. Localization Of Nuclear Processes. 20. Mapping Of Dna Replication Sites In Situ By Fluorescence Microscopy -- 21. Em Visualization Of Transcriptionally Active Genes After Injection Into Xenopus Oocyte Nuclei -- 22. Cell-free Systems To Study Chromatin Remodeling -- 23. In Vitro Systems For The Reconstitution Of Snrnp And Protein Nuclear Import -- 24. In Vivo Nuclear Transport Kinetics In Saccharomyces Cerevisiae -- 25. Nuclear Transport Of Rnas In Microinjected Xenopus Oocytes -- 26. In Vivo Systems To Study The Dynamics Of Nuclear Lamins. Edited By Miguel Berrios. Includes Bibliographical References And Index.