HPLC of Macromolecules: A Practical Approach (Practical Approach Series)

HPLC stands for high pressure (or performance) liquid chromatography, and is a standard biochemical technique for separating molecules. This volume covers the larger biomolecules—oligosaccharides, glycopeptides, oligonucleotides, polypeptides, and proteins—and includes the latest advances in microbore and packed capillary technology, and in the use of mass spectrometric detection.

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This revised edition translates recent innovations in high-performance liquid chromotography (HPLC) into practical laboratory techniques. Two new chapters open the work with discussions of microbore and packed capillary columns and mass spectrometric detectors. Other topics include size-exclusion HPLC of proteins; ion-exchange chromatography of proteins and peptides; reversed-phase chromatography of proteins; HPLC of oligonucleotides and polynucleotides; and HPLC of oligosaccharides and glycopeptides. For postgraduate students and researchers in biochemistry, biomedical sciences, and chemistry. Distributed by Oxford University Press. Annotation c. Book News, Inc., Portland, OR (booknews.com)