Guide to Protein Purification, Volume 182: Volume 182: Guide to Protein Purification (Methods in Enzymology)

Why Purify Enzymes / Arthur Kornberg -- Strategies And Considerations For Protein Purifications / Stuart Linn -- Setting Up A Laboratory / Murray P. Deutscher -- Buffers: Principles And Practice / Vincent S. Stoll, John S. Blanchard -- Measurement Of Enzyme Activity / Edward F. Rossomando -- Quantitation Of Protein / Christa M. Stoscheck -- Concentration Of Proteins And Removal Of Solutes / Thomas Pohl -- Maintaining Protein Stability / Murray P. Deutscher -- Overproduction Of Proteins In Escherichia Coli: Vectors, Hosts, And Strategies / Asis Das -- Overexpression Of Proteins In Eukaryotes / Margaret K. Bradley -- Secretion Of Heterologous Proteins In Escherichia Coli / I. Barry Holland, Brendan Kenny, Boris Steipe, Andreas Plückthun -- Preparation Of Extracts From Prokaryotes / Millard Cull, Charles S. Mchenry -- Preparation Of Extracts From Yeast / S. Michal Jazwinski -- Preparation Of Extracts From Plants / Peter Gegenheimer --^ Preparation Of Extracts From Higher Eukaryotes / John David Dignam -- Isolation Of Subcellular Organelles / Brian Storrie, Edward A Madden -- Preparation Of Membrane Fractions / Juris Ozols -- Detergents: An Overview / Judith M. Neugebauer -- Solubilization Of Native Membrane Proteins / Leonard M. Hjelmeland -- Solubilization Of Protein Aggregates / Fiona A.o. Marston, Donna L. Hartley -- Removal Of Detergents From Membrane Proteins / Leonard M. Hjelmeland -- Precipitation Techniques / Sasha Englard, Sam Seifter -- Precipitation Of Proteins With Polyethylene Glycol / Kenneth C. Ingham -- Ion-exchange Chromatography / Edward F. Rossomando -- Gel Filtration / Earle Stellwagen -- Protein Chromatography On Hydroxyapatite Columns / Marina J. Gorbunoff -- Hydrophobic Chromatography / Robert M. Kennedy -- Chromatography On Immobilized Reactive Dyes / Earle Stellwagen -- Affinity Chromatography: General Methods / Steven Ostrove --^ Affinity Chromatography: Specialized Techniques / Steven Ostrove, Shelly Weiss -- Chromatofocusing / Lallan Giri -- High-performance Liquid Chromatography: Effective Protein Purification By Various Chromatographic Modes / Roman M. Chicz, Fred E. Regnier -- One-dimensional Gel Electrophoresis / David E. Garfin -- Protein Analysis Using High-resolution Two-dimensional Polyacrylamide Gel Electrophoresis / Bonnie S. Dunbar, Hitomi Kimura, Therese M. Timmons -- Isoelectric Focusing / David E. Garfin -- Gel-staining Techniques / Carl R. Merril -- Elution Of Protein From Gels / Michael G. Harrington -- Purification Of Membrane Proteins / Thomas C. Thomas, Mark G. Mcnamee -- Purification Of Dna-binding Proteins By Site-specific Dna Affinity Chromatography / Shlomo Eisenberg, Stephen C. Francesconi, Chris Civalier, Scott S. Walker -- Purification Of Glycoproteins / Craig Gerard -- Purification Of Multienzyme Complexes / Paul A. Srere, Christopher K. Mathews --^ Determination Of Purity / David G. Rhodes, Thomas M. Laue -- Determination Of Size, Molecular Weight, And Presence Of Subunits / Thomas M. Laue, David G. Rhodes -- Amino Acid Analysis / Juris Ozols -- Limited N-terminal Sequence Analysis / Paul Matsudaira -- Peptide Mapping / Ralph C. Judd -- Analysis For Protein Modifications And Nonprotein Cofactors / Sam Seifter, Sasha Englard -- Protein Crystallization / David Ollis, Stephen White -- Preparation Of Polyclonal Antibodies / Bonnie S. Dunbar, Eric D. Schwoebel -- Preparation Of Monoclonal Antibodies / Bonnie S. Dunbar, Sheri M. Skinner -- Protein Blotting And Immunodetection / Therese M. Timmons, Bonnie S. Dunbar -- Immunoprecipitation Of Proteins / Gary L. Firestone, Sandra D. Winguth -- Immunoassays / Charles W. Parker -- Radiolabeling Of Proteins / Charles W. Parker -- Using Purified Protein To Clone Its Gene / John M. Wozney -- Computer Analysis Of Protein Structure / Patrick Argos --^ Rethinking Your Purification Procedure / Murray P. Deutscher -- The Private Life Of Dna Polymerase I / Arthur Kornberg -- Purification And Crystallization Of Transaldolase From Candida Utilis / B.l. Horecker, O. Tsolas -- Discovery Of Glutamine Synthetase Cascade / Earl R. Stadtman -- Discovery, Resolution, Purification, And Function Of Elongation Factors / Kivie Moldave Edited By Murray P. Deutscher. Includes Bibliographical References.