DNA Cloning: A Practical Approach Volume 4: Mammalian Systems (The Practical Approach Series, 164)

In recent years, heterologous gene expression in mammalian cells has become invaluable for studying many aspects of eukaryote cell and molecular biology. This book provides key background information and detailed practical guidance on using the major expression systems currently available. The experienced authors describe laboratory protocols that they know work, and give clear technical advice, hints and tips, troubleshooting comments, and example results, all designed to ensure practical success. Topics covered include: high-efficiency gene transfer into mammalian cells; construction and characterization of vaccinia virus recombinants; use of vectors based on gene amplification; retroviral vectors; genetic manipulation of embryonic stem cells; production of transgenic rodents by microinjection of cloned DNA; genomic and expression analysis of transgenic animals; expression using a defective HSV-1 vector system; adenovirus vectors. This volume, Mammalian Systems, is one of four new Practical Approach volumes on DNA cloning which together provide a comprehensive practical manual of current recombinant DNA methodology. Mammalian Systems will prove invaluable to all researchers, from PhD students to experienced group leaders, who already express heterologous genes in mammalian systems or who plan to do so.

The book contains black-and-white illustrations.