Contemporary Enzyme Kinetics and Mechanism: Selected Methods in Enzymology

Transient Kinetic Approaches To Enzyme Mechanisms / Carol A. Fierke -- Practical Considerations In The Design Of Initial Velocity Enzyme Rate Assays / R. Donald Allison -- Derivation Of Initial Velocity And Isotope Exchange Rate Equations / Charles Y. Huang -- Plotting Methods For Analyzing Enzyme Rate Data / Frederick B. Rudolph -- Regression Analysis, Experimental Error, And Statistical Criteria In The Design And Analysis Of Experiments For Discrimination Between Rival Kinetic Models / Bengt Mannervik -- Cooperativity In Enzyme Function: Equilibrium And Kinetic Aspects / Kenneth E. Neet -- Product Inhibition Applications / Bruce F. Cooper -- Reversible Enzyme Inhibitors As Mechanistic Probes / Herbert J. Fromm -- Transition State And Multisubstrate Analog Inhibitors / Anna Radzicka -- Application Of Affinity Labeling For Studying Structure And Function Of Enzymes / Bryce V. Plapp -- Mechanism-based Enzyme Inactivators / Richard B. Silverman -- Isotope Exchange Methods For Elucidating Enzymatic Catalysis / Daniel L. Purich -- Positional Isotope Exchange As Probe Of Enzyme Action / Leisha S. Mullins -- Isotope Effects: Determination Of Enzyme Transitional State Structure / W.w. Cleland -- Hydrogen Tunneling In Enzyme Catalysis / Brian J. Bahnson -- Site-directed Mutagenesis: A Tool For Studying Enzyme Catalysis / Bryce V. Plapp -- Selected Exercises And Problems / Daniel L. Purich. Edited By Daniel L. Purich. Includes Bibliographical References And Index.