Chromatin: A Practical Approach (practical Approach Series)

Developments in the analysis of the regulation of chromatin structure have provided much information on their relevance in the control of DNA replication and transcription, making it an important area of research. Chromatin: A Practical Approach provides clear explanations of a wide range of techniques from the initial isolation and fractionation of chromatin and linker histones, to the in vitro assembly of chromatin and nuclear structures in Xenopus egg extracts. Both in vivo and in vitro methods are covered and clear explanations of procedures are provided throughout making this volume an invaluable aid to laboratory workers in biochemistry and molecular biology, especially those whose research encompasses eukaryotic transcription, DNA replication, and chromosome structure.

Leo Miller

This book, part of The Practical Approach Series, provides a thorough, up-to-date explanation of techniques used to study chromatin. It includes well-organized methods on fractionation, assembly, mapping, reconstitution, and separation of the constituent components of chromatin. The purpose is to provide a one-stop resource for the numerous techniques used to study chromatin. Methods for structural studies, for example, range from electron microscopy to in vivo footprinting. This book is aimed at researchers interested in the biochemistry, molecular biology, and developmental aspects of chromatin structure and function. The different chapters have a good balance of black-and-white illustrations. An extensive reference section and an appendix with a list of suppliers are included. This volume is highly recommended for libraries. It will be very useful to all researchers who study chromatin structure and function. Everything from the most basic methods for the isolation of nuclei to the newest techniques for in vivo footprinting using ligation mediated polymerase chain reaction is included.